AustralieFrV1, Main, Exploration, bibRecord, 006665

The Arabidopsis thaliana 2-D gel mitochondrial proteome: Refining the value of reference maps for assessing protein abundance, contaminants and post-translational modifications

Identifieur interne : 006665 ( Main/Exploration ); précédent : 006664; suivant : 006666

The Arabidopsis thaliana 2-D gel mitochondrial proteome: Refining the value of reference maps for assessing protein abundance, contaminants and post-translational modifications

Auteurs : Nicolas L. Taylor [Australie] ; Joshua L. Heazlewood [Australie, États-Unis] ; A. Harvey Millar [Australie]

Source :

Proteomics : (Weinheim. Print) [ 1615-9853 ] ; 2011.

RBID : Pascal:11-0277606

Descripteurs français

Pascal (Inist)
- Arabidopsis thaliana, Mitochondrie, Protéome, Référence, Protéine, Modification posttraductionnelle, Protéomique, Phosphorylation.

English descriptors

KwdEn :
- Arabidopsis thaliana, Mitochondria, Phosphorylation, Posttranslational modification, Protein, Proteome, Proteomics, Reference.

Abstract

Mitochondria undertake the process of oxidative phosphorylation yielding ATP for plant cell maintenance and growth. The principles of isolation and fractionation of plant mitochondrial proteins have been improved over decades, and surveys of the mitochondrial proteome in a number of plants species have been performed. Over time, many quantitative analyses of changes in the plant mitochondrial proteome have been performed by 2-D gel analyses revealing the induction, degradation and modification of mitochondrial proteins in responses to mutation, stress and development. Here, we present a saturating MS analysis of 2-D gel separable protein spots from a typical purification of Arabidopsis mitochondria identifying 264 proteins, alongside an LC-MS/MS survey by non-gel methods identifying 220 proteins. This allowed us to characterise the major mitochondrial proteins that are not observed on 2-D gels, the common contaminants and the abundance of the protein machinery of key mitochondrial biochemical pathways, and consider the impact of N-terminal pre-sequence cleavage and phosphorylation as explanations of multiple protein spots and the co-ordinates of proteins on 2-D gels.

Affiliations:

Australie, États-Unis

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Le document en format XML

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The Arabidopsis thaliana 2-D gel mitochondrial proteome: Refining the value of reference maps for assessing protein abundance, contaminants and post-translational modifications

The Arabidopsis thaliana 2-D gel mitochondrial proteome: Refining the value of reference maps for assessing protein abundance, contaminants and post-translational modifications

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri